polyclonal rabbit anti human arhgap29 receptor antiserum  (Novus Biologicals)

Journal: Cancer Genomics & Proteomics

Article Title: ARHGAP29 Is Involved in Increased Invasiveness of Tamoxifen-resistant Breast Cancer Cells and its Expression Levels Correlate With Clinical Tumor Parameters of Breast Cancer Patients

doi: 10.21873/cgp.20454

Figure Lengend Snippet: Information on tissue of origin, pathology, grading, staging, TNM classification and assessment of ARHGAP29 expression. (–) not expressed, (+) slightly expressed, (++) moderately expressed and (+++) strongly expressed.

Article Snippet: Antigens were retrieved by incubation with 0.01 M citrate buffer (pH 6.0) in a microwave (700 W) for 5 min. Endogenous peroxidase activity was quenched by treatment with 3% hydrogen peroxide solution for 6 min. After washing in PBS, the slides were treated with polyclonal rabbit anti-human ARHGAP29 receptor antiserum (#NBP1-05989, Novus Biologicals, Centennial, CO, USA) in a 1:50 dilution in 1% BSA in 10 mM Tris, pH 8.0, 500 mM NaCl and 0.1% Tween-20 (TBST) over night.

Techniques: Expressing

Journal: Frontiers in Genetics

Article Title: Targeted re-sequencing on 1p22 among non-syndromic orofacial clefts from Han Chinese population

doi: 10.3389/fgene.2022.947126

Figure Lengend Snippet: (A) Sanger sequencing results of the de novo harmful rare variant in ARHGAP29 . Sequence chromatograms indicate the heterozygous variant (NM_004815.3, NP_004806.3; c.1652G>C, p.R551T). The red letter and box emphasize the cross-species conservation of the altered amino acid. (B, C) Western blot and RT-qPCR analysis of the ARHGAP29 expression in HEK-293T cells 48 h after plasmid transfection. The results are presented as mean values with standard deviation (SD) normalized to GAPDH , and there were three biological replicates, *** p < 0.001.

Article Snippet: Subsequently, protein samples were separated by electrophoresis in agarose gels and transferred onto PVDF membranes, which were then blocked by 5% milk for 1 h and incubated with rabbit anti-human Arhgap29 antibody (Novus Biologicals, United States) at 4°C overnight, followed by incubation with anti-rabbit antibody (Proteintech, China) at room temperature for 1 h. At last, proteins were visualized by ECL substrate (Epizyme, China).

Techniques: Sequencing, Variant Assay, Western Blot, Quantitative RT-PCR, Expressing, Plasmid Preparation, Transfection, Standard Deviation

Journal: Frontiers in Genetics

Article Title: Targeted re-sequencing on 1p22 among non-syndromic orofacial clefts from Han Chinese population

doi: 10.3389/fgene.2022.947126

Figure Lengend Snippet: Results of RNA sequencing on Arhgap29 R553T/R553T and wild-type mice. (A) Volcanic maps of differential expression genes. (B) GO analysis of DEGs. All the shown GO terms were significantly enriched with Q-value less than 0.05.

Article Snippet: Subsequently, protein samples were separated by electrophoresis in agarose gels and transferred onto PVDF membranes, which were then blocked by 5% milk for 1 h and incubated with rabbit anti-human Arhgap29 antibody (Novus Biologicals, United States) at 4°C overnight, followed by incubation with anti-rabbit antibody (Proteintech, China) at room temperature for 1 h. At last, proteins were visualized by ECL substrate (Epizyme, China).

Techniques: RNA Sequencing, Quantitative Proteomics